Fertilization occurs in the ampullary part of fallopian tube so zygote is formed in the ampullary part of fallopian tube. Immediately after its formation it starts: 1. Moving towards uterus 2. Multiplying mitotically Zygote immediately after its formation, in the ampullary part, is guided medially through the uterine tube toward the uterine cavity. As the zygote, passes through the uterine tube, it undergoes mitotic cell divisions known as cleavage. This phase of development begins with the first mitotic division of the zygote and ends with formation of blastocyst. It extends for 6 to 7 days or a week. The 1st division of zygote gives rise to two daughter cells. They are called blastomeres. Each blastomere is half the size of parent cell. They develop about 30 hours after fertilization. Subsequent divisions follow one another, forming progressively smaller blastomeres. After three or four divisions, the zygote, looks like a mulberry and is known as morula (L. Morus, mulberry). It is a solid mass of 12 to 16 cells (blastomeres). This stage is reached about three days after fertilization and the mass of cells is about to enter the uterus. At this time, the morula consists of a group of centrally located cells, the inner cell mass, and a surrounding layer, the outer cell mass. The inner cell mass will give rise to the tissues of the embryo proper, while the outer cell mass forms the trophoblast which later contributes in the formation of placenta and other membranes. Note also the degeneration process of zona pellucida. Morula enters uterine cavity about 4th day after fertilization. As the morula enters the uterine cavity fluid begins to penetrate through the zona pellucida. Spaces appear between the inner cell mass and outer cell mass. During the formation of blastocele the cells of outer cell mass becomes flattened. So the outer cell mass is now a single layer of flattened cells forming the wall of blastocyst and it is now given the name trophoblast (Gr. Trophe, nutrition), which will form placenta and associated membranes. The cells of the inner cell mass are attached to one side of trophoblast and project into the blastocyst cavity. They are now referred to as embryoblast, which will form the embryo. The zygote lies free in the uterine secretions for about two days. During this time the zona pellucida degenerates and disappears. The blastocyst hatches out and this is called hatching of blastocyst. At about 6th day after fertilization the embryonic pole of the blastocyst becomes attached to the endometrium and start penetrating into it. Zona pellucida Zona pellucida is created around primary oocyte when primordial follicle is transformed into primary follicle. So Zona Pellucida is not present in primordial follicle while primary follicle is characterized by Zona Pellucida. This Zona Pellucida is present in all follicles (primary follicles, secondary follicles, tertiary follicles and Graafian follicles) except primordial follicles. Zona Pellucida surrounds primary oocyte and itself Zona Pellucida is surrounded by granulosa cells. Just before ovulation the primary oocyte is converted into secondary oocyte and it is always the secondary oocyte, which ovulates. Now the Zona Pellucida contains secondary oocyte and first polar body. After ovulation the granulosa cells surrounding the secondary oocyte and zona pellucida are given the name corona radiata cells. During fertilization the sperms after passing through corona radiata, become attached to Zona Pellucida. The enzymes released from the acrosome (esterases, acrosin, and neuraminidase) causes lysis of Zona Pellucida, thereby forming a path for the sperm through Zona Pellucida. After fertilization Zona Pellucida contains ovum with two polar bodies. Zona pellucida remains present around zygote and morula at different stages of development. Shortly after the morula enters the uterus (about 4 days after fertilization), fluid starts appearing within the morula between the blastomeres. This fluid passes from the uterine cavity through the zona pellucida to form spaces.